Estudo da interação da enzima tripsina com nanopartículas de ouro

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Universidade Federal de Goiás


Proteases are one of the largest groups of enzymes and have several applications ranging from food processes to pharmaceuticals. Within this group, Trypsin, a serine protease excreted by the pancreas as an inactive, proenzyme (trypsinogen), is present in the digestive systems of vertebrates and invertebrates. As protease is one of the most abundant classes of enzymes, there are several studies on its physiological importance. Some studies show, for example, the significant role of the interaction of nanomaterials with enzymatic biomolecules, such as proteases. In comparison with small molecules, nanoscale structures express several physical and chemical properties, which can be associated with biomolecules in order to enhance their functions. One of the most used nanomaterials are gold nanoparticles (NpAu), which are produced for various applications, for example, in therapeutics, detection and diagnosis, chemical and biological sensing and catalysis fields. Within this context, this work reports two routes of synthesis of gold nanoparticles associated with the enzyme Trypsin. The synthesis methodology was based on the traditional Turkevich method followed by adaptations. The results showed that the two methodologies were efficient, generating the expected biconjugate systems, as can be proved by the spectroscopy analyzes in the UV-Vis region. The infrared (FTIR) confirmed the interactions between the enzyme and the surface of the gold nanoparticle. Morphologically, the images obtained by scanning electron microscopy (SEM) transmission (TEM) showed nanoparticles spherical and without agglomerations. The gold nanoparticle and trypsin biosystem proved to be stable over the 30-day period. This biosystem is promising for the understanding of future applications that involve the interaction process between the enzyme and its respective substrate.



Nanopartículas de ouro, Tripsina, PEG, Gold nanoparticles, Trypsin, PEG


SANTOS, L. A. Estudo da interação da enzima tripsina com nanopartículas de ouro. 2020. 67 f. Dissertação (Mestrado em Química) - Universidade Federal de Goiás, Catalão, 2020.