Estudo da interação da enzima tripsina com nanopartículas de ouro
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Data
2020-07-03
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Universidade Federal de Goiás
Resumo
Proteases are one of the largest groups of enzymes and have several applications ranging
from food processes to pharmaceuticals. Within this group, Trypsin, a serine protease
excreted by the pancreas as an inactive, proenzyme (trypsinogen), is present in the digestive
systems of vertebrates and invertebrates. As protease is one of the most abundant classes of
enzymes, there are several studies on its physiological importance. Some studies show, for
example, the significant role of the interaction of nanomaterials with enzymatic biomolecules,
such as proteases. In comparison with small molecules, nanoscale structures express several
physical and chemical properties, which can be associated with biomolecules in order to
enhance their functions. One of the most used nanomaterials are gold nanoparticles (NpAu),
which are produced for various applications, for example, in therapeutics, detection and
diagnosis, chemical and biological sensing and catalysis fields. Within this context, this work
reports two routes of synthesis of gold nanoparticles associated with the enzyme Trypsin. The
synthesis methodology was based on the traditional Turkevich method followed by
adaptations. The results showed that the two methodologies were efficient, generating the
expected biconjugate systems, as can be proved by the spectroscopy analyzes in the UV-Vis
region. The infrared (FTIR) confirmed the interactions between the enzyme and the surface of
the gold nanoparticle. Morphologically, the images obtained by scanning electron microscopy
(SEM) transmission (TEM) showed nanoparticles spherical and without agglomerations. The
gold nanoparticle and trypsin biosystem proved to be stable over the 30-day period. This
biosystem is promising for the understanding of future applications that involve the
interaction process between the enzyme and its respective substrate.
Descrição
Palavras-chave
Nanopartículas de ouro, Tripsina, PEG, Gold nanoparticles, Trypsin, PEG
Citação
SANTOS, L. A. Estudo da interação da enzima tripsina com nanopartículas de ouro. 2020. 67 f. Dissertação (Mestrado em Química) - Universidade Federal de Goiás, Catalão, 2020.