Estudo da interação entre a enzima tripsina e nanopartículas de prata

Abstract

The study on interactions between biomolecules and nanomaterials is a chemistry/nanoscience in- terface that emerges to understand the behavior of materials in relation of several biomolecules, since this materials interact with biologic fluids, the study of how these nanomaterials and biomo- lecules interactions happen became an important task. Enzymes play a fundamental role in metabo- lism and their imbalance can lead to the onset or worsening of diseases. The enzyme trypsin is the most used enzyme for over five decades due to its physiological importance, as it participates in the digestive process, blood clotting and fertilization. In this context, this work aimed to study how the interaction between the enzyme trypsin and silver nanoparticles occurs in aqueous medium. Sil- ver nanoparticles were synthesized using the chemical co-reduction method, which presented the plasmatic band with maximum absorption at 407 nm, after the addition of trypsin for adsorption, which caused a shift to the color red. Through spectroscopy in the infrared region, the presence of the main vibrational modes can be confirmed before and after the formation of the nanobiocompo- site. Morphologically, the synthesized nanoparticles had a spherical shape. Other studies revealed that, after the interaction, there is a zeta potential decrease and a consequent increase in the hydro- dynamic radius, these changes in the characteristics affected the secondary structure of the protein, causing loss of organization and formation of a more hydrophobic nanoparticleenzyme complex, due to the interactions electrostatic and hydrogen bonding.